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The knowledge base and Yua say to use D4 for Game Boy games, but the drop in quality seems immense. I would really like to make a higher resolution encode with the D1 setting, as filesize is not much of an issue nowadays, but I figured I should bring the topic up here first.

For reference, D4: https://files.catbox.moe/gdp20k.mp4
D1: https://files.catbox.moe/eixzoa.mp4
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up to you. it's true that d1 will preserve more color information in qualities below IQ which have to use the yv12 (4:2:0) colorspace for compatibility reasons.